7XJT

Catabolic ornithine carbamoyltransferases (OTCs) from Psychrobacter sp. PAMC 21119

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-04-13
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9794
Spacegroup name	H 3
Unit cell lengths	130.061, 130.061, 329.326
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.527 - 2.598
Rwork	0.201
R-free	0.26420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	modeled
RMSD bond length	0.009
RMSD bond angle	1.721
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.527	2.650
High resolution limit [Å]	2.598	2.598
Number of reflections	63985	3218
<I/σ(I)>	31.3
Completeness [%]	99.9
Redundancy	5.3
CC(1/2)	0.995	0.959

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1M Tris-HCl pH 8.1, 1.3M Lithium Sulfate