7XJE
Crystal structure of bacteriorhodopsin in the K state refined against the extrapolated dataset
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 15 |
Detector technology | PIXEL |
Collection date | 2020-10-06 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.8 |
Spacegroup name | P 63 |
Unit cell lengths | 60.570, 60.570, 110.880 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 1.330 |
Rwork | 0.274 |
R-free | 0.29300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | D_1300028945 |
Phasing software | CNS |
Refinement software | SHELX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.400 |
High resolution limit [Å] | 1.330 | 1.330 |
Number of reflections | 290824 | 44229 |
<I/σ(I)> | 10.08 | 1.24 |
Completeness [%] | 99.9 | |
Redundancy | 5.8 | |
CC(1/2) | 0.990 | 0.430 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 293 | MO, 2.0-2.5 M PHOSPHATE |