7XIV

Structural insight into the interactions between Lloviu virus VP30 and nucleoprotein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-13
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97915
Spacegroup name	P 43 21 2
Unit cell lengths	64.251, 64.251, 175.795
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.126 - 2.498
R-factor	0.233
Rwork	0.229
R-free	0.26940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5dvw
RMSD bond length	0.010
RMSD bond angle	1.298
Data reduction software	MOSFLM
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.126	2.598
High resolution limit [Å]	2.498	2.498
Rpim	0.020	0.084
Number of reflections	13571	660
<I/σ(I)>	40.4
Completeness [%]	100.0
Redundancy	13.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.1 M Tris-HCl, pH 7.0, 0.25 M NaCl, 22% (w/v) polyethylene glycol 3350