7XHR
Crystal structure of Wild Type Cypovirus Polyhedra produced by cell-free protein synthesis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL32XU |
Synchrotron site | SPring-8 |
Beamline | BL32XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-12-04 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1 |
Spacegroup name | I 2 3 |
Unit cell lengths | 103.610, 103.610, 103.610 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.299 - 1.801 |
Rwork | 0.149 |
R-free | 0.18400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5gqm |
RMSD bond length | 0.009 |
RMSD bond angle | 1.530 |
Data reduction software | XDS |
Data scaling software | CrystFEL |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.810 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 17297 | 414 |
<I/σ(I)> | 5.5 | 1.53 |
Completeness [%] | 100.0 | |
Redundancy | 399 | |
CC(1/2) | 0.990 | 0.533 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | SMALL TUBES | 293 | cell-free crystallization |