7XH0
crystal structure of Csn-PD from Paenibacillus dendritiformis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-08-27 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.18071 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 68.510, 73.138, 99.975 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.720 - 1.680 |
| Rwork | 0.150 |
| R-free | 0.19510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2d05 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.639 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.720 | 1.710 |
| High resolution limit [Å] | 1.680 | 1.680 |
| Rmerge | 0.052 | 0.446 |
| Number of reflections | 28796 | 1417 |
| <I/σ(I)> | 21.7 | |
| Completeness [%] | 99.4 | |
| Redundancy | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M Citric acid pH3.5, 25% PEG3,350 |
