7XGW
Apo structure of LW domain from Trypanosoma brucei
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-03-16 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9791 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 111.724, 111.724, 93.970 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.265 - 2.250 |
R-factor | 0.2025 |
Rwork | 0.199 |
R-free | 0.22990 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7fax |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | REFMAC |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.380 | 2.320 |
High resolution limit [Å] | 2.250 | 2.250 |
Rmerge | 0.101 | 1.111 |
Number of reflections | 32354 | 2857 |
<I/σ(I)> | 19.5 | |
Completeness [%] | 99.4 | |
Redundancy | 19.1 | |
CC(1/2) | 0.999 | 0.798 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 283 | 1.5M Lithium sulfate monohydrate, 0.1M Tris pH 8.5 |