7XGS

Short vegetative phase protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	100
Detector technology	CCD
Collection date	2018-03-03
Detector	RAYONIX MX300-HS
Wavelength(s)	1.0000
Spacegroup name	C 2 2 2
Unit cell lengths	42.617, 116.440, 50.274
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.210
R-factor	0.1904
Rwork	0.188
R-free	0.23320
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.531
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	BUCCANEER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.039	0.285
Number of reflections	6460	598
<I/σ(I)>	52.7	5.7
Completeness [%]	98.6
Redundancy	9.1	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	Na-citrate, PEG 8000, ethylene glycol