7XGQ
Crystal structure of chitosanase crystallized by ammonium sulfate with glycan
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-02-22 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.422, 92.110, 175.169 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.729 - 1.500 |
| R-factor | 0.1831 |
| Rwork | 0.182 |
| R-free | 0.21000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7cju |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.1.27) |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.13) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.730 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.056 | 0.975 |
| Rmeas | 0.061 | 1.055 |
| Rpim | 0.023 | 0.397 |
| Number of reflections | 142580 | 6809 |
| <I/σ(I)> | 18.8 | |
| Completeness [%] | 95.7 | 93.1 |
| Redundancy | 6.8 | 6.9 |
| CC(1/2) | 0.991 | 0.559 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 291 | 0.1M Sodium Citrate, 3.4M Ammonium Sulfate, 5%(v/v) tri[(O-beta-D-glucopyranosyl)ethyloxy]cholane |
