7XGL

Quinolinate Phosphoribosyl Transferase (QAPRTase) from Streptomyces pyridomyceticus NRRL B-2517 in Apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	193
Detector technology	PIXEL
Collection date	2020-06-18
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97915
Spacegroup name	P 21 21 2
Unit cell lengths	120.046, 150.929, 55.181
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.730 - 2.110
R-factor	0.178
Rwork	0.176
R-free	0.20730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qpo
RMSD bond length	0.007
RMSD bond angle	0.817
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	MOLREP
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.730	2.140
High resolution limit [Å]	2.110	2.110
Number of reflections	58249	5409
<I/σ(I)>	11.1
Completeness [%]	99.4
Redundancy	6.5
CC(1/2)	0.993	0.838

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293	0.1M Citric acid, pH 5.0, 1.6M Ammonium sulfate