7XGC
Crystal structure of influenza polymerase acidic subunit N-terminal domain crystallized by ammonium sulfate with glycan
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-12-17 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 66.177, 66.177, 125.727 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.536 - 1.600 |
| R-factor | 0.2063 |
| Rwork | 0.205 |
| R-free | 0.22790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zqq |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.1.27) |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.13) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.790 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.047 | 1.174 |
| Rmeas | 0.049 | 1.282 |
| Rpim | 0.014 | 0.495 |
| Number of reflections | 37623 | 1755 |
| <I/σ(I)> | 23 | |
| Completeness [%] | 99.7 | 96.4 |
| Redundancy | 11.8 | 6.2 |
| CC(1/2) | 1.000 | 0.522 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 291 | 0.1M MES, 1.275M Ammonium Sulfate, 0.1M POTASSIUM CHLORIDE, 9%(v/v) TREHALOSE |
