7XGB
Crystal structure of the ctcP from Streptomyces aureofaciens
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-01-01 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97923 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 116.393, 121.281, 179.194 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.800 - 2.700 |
R-factor | 0.1777 |
Rwork | 0.176 |
R-free | 0.22430 |
Structure solution method | SAD |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.800 |
High resolution limit [Å] | 2.700 | 5.810 | 2.700 |
Rmerge | 0.177 | 0.082 | 0.812 |
Total number of observations | 1038277 | ||
Number of reflections | 70271 | 7381 | 6909 |
<I/σ(I)> | 8.1 | ||
Completeness [%] | 100.0 | 100 | 100 |
Redundancy | 14.8 | 14 | 14.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1M BIS-TRIS pH 6.0, 0.2M Magnesium sulfate heptahydrate, 20% w/v Polyethylene glycol 3350 |