7XG9
Crystal structural of methylenetetrahydrofolate reductase from Sphingobium sp. SYK-6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 95 |
| Detector technology | PIXEL |
| Collection date | 2018-02-25 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 2.7 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.056, 168.117, 45.424 |
| Unit cell angles | 90.00, 105.55, 90.00 |
Refinement procedure
| Resolution | 34.920 - 1.500 |
| R-factor | 0.1812 |
| Rwork | 0.180 |
| R-free | 0.20360 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.842 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | CRANK2 |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.350 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.034 | 0.239 |
| Rmeas | 0.046 | 0.322 |
| Rpim | 0.031 | 0.215 |
| Number of reflections | 84735 | 4091 |
| <I/σ(I)> | 19.1 | 4.4 |
| Completeness [%] | 99.5 | 96.3 |
| Redundancy | 3.7 | 3.1 |
| CC(1/2) | 0.999 | 0.913 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 277 | 20% (w/v) PEG 4000, 0.1 M sodium citrate pH 4.5 |
