7XFS
MucP PDZ1 domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-14 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 21 2 21 |
| Unit cell lengths | 34.640, 49.466, 100.245 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.470 - 1.060 |
| R-factor | 0.151 |
| Rwork | 0.150 |
| R-free | 0.17340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3id1 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.942 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.080 |
| High resolution limit [Å] | 1.060 | 1.060 |
| Rpim | 0.024 | 0.175 |
| Number of reflections | 78318 | 3570 |
| <I/σ(I)> | 30.5 | 3.5 |
| Completeness [%] | 98.8 | 91.2 |
| Redundancy | 6.1 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 40% PEG 300, 0.1M sodium arsenate pH 6.5, 0.2M calcium acetate |
