7XF1

Crystal strucutre of apoCasDinG in complex with ssDNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17B1
Synchrotron site	SSRF
Beamline	BL17B1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97920
Spacegroup name	P 32 2 1
Unit cell lengths	111.181, 111.181, 152.736
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.460 - 3.200
R-factor	0.2089
Rwork	0.207
R-free	0.23550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6fwr
Data scaling software	HKL-3000
Refinement software	PHENIX (1.20.1-4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	3.310
High resolution limit [Å]	3.200	3.200
Number of reflections	18489	1797
<I/σ(I)>	7
Completeness [%]	99.8
Redundancy	18
CC(1/2)	0.984	0.575

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289.15	0.1M MES pH 6.0, 1.6M Ammonium sulfate