7XF0
Crystal strucutre of CasDinG in complex with ATP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-09-21 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 135.546, 72.095, 224.230 |
| Unit cell angles | 90.00, 90.84, 90.00 |
Refinement procedure
| Resolution | 38.910 - 3.100 |
| R-factor | 0.2253 |
| Rwork | 0.224 |
| R-free | 0.26050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6fwr |
| Data scaling software | DIALS (0.7.1) |
| Refinement software | PHENIX (1.20.1-4487) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 38.910 | 38.910 | 3.230 |
| High resolution limit [Å] | 3.100 | 11.180 | 3.100 |
| Rmerge | 0.166 | 0.084 | 1.000 |
| Rmeas | 0.180 | 0.092 | 1.079 |
| Rpim | 0.069 | 0.036 | 0.403 |
| Total number of observations | 267256 | 5697 | 31362 |
| Number of reflections | 39721 | 889 | 4432 |
| <I/σ(I)> | 9 | 17 | 3 |
| Completeness [%] | 99.9 | 97.7 | 100 |
| Redundancy | 6.7 | 6.4 | 7.1 |
| CC(1/2) | 0.992 | 0.988 | 0.914 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289.15 | 8% PEG 6000, 0.1M HEPES 7.4 |
