7XED

Crystal Structure of OsCIE1-Ubox and OsUBC8 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-21
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.979
Spacegroup name	P 21 21 2
Unit cell lengths	87.290, 132.550, 47.340
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.640 - 2.500
R-factor	0.2329
Rwork	0.231
R-free	0.24940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2oxq
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	66.280	2.570
High resolution limit [Å]	2.500	2.500
Number of reflections	19672	1418
<I/σ(I)>	9.8
Completeness [%]	99.9	100
Redundancy	12.8
CC(1/2)	0.992	0.341

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.8	300	0.3M Calcium acetate, 20% PEG 3350, 0.1M MES