7XEA

T4 lysozyme mutant-S44C/C54T/N68C/A93C/C97A/T115C, DMSO 40%, and then backsoaking

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Collection date	2007-11-06
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	P 32 2 1
Unit cell lengths	59.898, 59.898, 96.770
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.050 - 1.100
R-factor	0.1595
Rwork	0.159
R-free	0.18130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5vnr
RMSD bond length	0.006
RMSD bond angle	0.938
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.400	1.140
High resolution limit [Å]	1.100	1.100
Rmeas	0.062	0.630
Number of reflections	81025	7157
<I/σ(I)>	23.3	2.1
Completeness [%]	98.7	88.4
Redundancy	9.8	5.5
CC(1/2)	0.999	0.812

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	A crystal was soaked into the precipitant-free solutions containing 40% DMSO, and then backsoaked into the precipitant-free solution.