7XE7
T4 lysozyme mutant-S44C/C54T/N68C/A93C/C97A/T115C, pH10
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-01-25 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.0 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 59.976, 59.976, 97.066 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.970 - 1.050 |
R-factor | 0.1645 |
Rwork | 0.164 |
R-free | 0.17780 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5vnr |
RMSD bond length | 0.005 |
RMSD bond angle | 0.968 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.000 | 1.090 |
High resolution limit [Å] | 1.050 | 1.050 |
Rmeas | 0.054 | 0.401 |
Number of reflections | 93665 | 8688 |
<I/σ(I)> | 27 | 3.1 |
Completeness [%] | 98.8 | 92.5 |
Redundancy | 10 | 6.4 |
CC(1/2) | 0.999 | 0.925 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | A crystal was soaked into precipitant-free solutions (pH10) |