7XDY
Crystal structure of a receptor like kinase from Arabidopsis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-07-11 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.979183 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.795, 83.248, 62.322 |
| Unit cell angles | 90.00, 93.04, 90.00 |
Refinement procedure
| Resolution | 61.708 - 2.280 |
| R-factor | 0.1996 |
| Rwork | 0.197 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qkw |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.15_3459) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.234 | 2.150 |
| High resolution limit [Å] | 1.957 | 1.960 |
| Rmerge | 0.101 | |
| Number of reflections | 32641 | 28740 |
| <I/σ(I)> | 8.3 | |
| Completeness [%] | 88.2 | |
| Redundancy | 3.3 | |
| CC(1/2) | 0.994 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1M Tris pH8.0, 30% v/v Polyethylene glycol 400 |
