7XDX
Crystal structure of a receptor like kinase from Arabidopsis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-07-11 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.979183 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 61.670, 82.950, 61.940 |
Unit cell angles | 90.00, 93.30, 90.00 |
Refinement procedure
Resolution | 61.568 - 2.230 |
R-factor | 0.1843 |
Rwork | 0.181 |
R-free | 0.23460 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2qkw |
Data reduction software | DIALS |
Data scaling software | DIALS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.15_3459) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 82.950 | 2.290 |
High resolution limit [Å] | 2.230 | 2.230 |
Rmerge | 0.111 | |
Number of reflections | 30350 | 28857 |
<I/σ(I)> | 9 | |
Completeness [%] | 99.5 | |
Redundancy | 3.2 | |
CC(1/2) | 0.982 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1M Tris pH 8.0, 30% v/v Polyethylene glycol 400 |