7XDW
Crystal structure of a receptor like kinase from Arabidopsis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-07-11 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.979183 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 61.805, 83.163, 62.048 |
Unit cell angles | 90.00, 93.14, 90.00 |
Refinement procedure
Resolution | 61.712 - 1.926 |
R-factor | 0.1877 |
Rwork | 0.187 |
R-free | 0.20740 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2qkw |
Data reduction software | autoPROC |
Data scaling software | autoPROC |
Phasing software | PHENIX |
Refinement software | PHENIX (1.15_3459) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 61.950 | 2.030 |
High resolution limit [Å] | 1.926 | 1.930 |
Rmerge | 0.057 | |
Number of reflections | 42862 | 42809 |
<I/σ(I)> | 13 | |
Completeness [%] | 90.2 | |
Redundancy | 3.3 | |
CC(1/2) | 0.997 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1M Potassium chloride, 0.01M Magnesium chloride hexahydrate, 0.05M TRIS hydrochloride pH 8.5, 30% v/v Polyethylene glycol 400 |