7XDV
Crystal structure of a receptor like kinase from Arabidopsis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-01-13 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.979183 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 61.950, 83.330, 62.050 |
Unit cell angles | 90.00, 93.39, 90.00 |
Refinement procedure
Resolution | 27.059 - 1.970 |
R-factor | 0.1723 |
Rwork | 0.171 |
R-free | 0.21300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2qkw |
Data reduction software | autoPROC |
Data scaling software | autoPROC |
Phasing software | PHENIX |
Refinement software | PHENIX (1.15_3459) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.760 | 2.020 |
High resolution limit [Å] | 1.970 | 1.970 |
Rmerge | 0.086 | |
Number of reflections | 44514 | 44474 |
<I/σ(I)> | 12.1 | |
Completeness [%] | 99.8 | |
Redundancy | 6.7 | |
CC(1/2) | 0.998 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.05M Magnesium chloride hexahydrate, 0.1M HEPES 7.5, 30% v/vPolyethylene glycol monomethyl ether 550 |