7XDQ

Crystal structure of a glucosylglycerol phosphorylase mutant from Marinobacter adhaerens

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	H 3 2
Unit cell lengths	176.437, 176.437, 128.473
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	21.520 - 2.830
R-factor	0.2473
Rwork	0.244
R-free	0.30220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6s9v
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.920
High resolution limit [Å]	2.820	2.820
Rpim	0.031	0.435
Number of reflections	18371	1800
<I/σ(I)>	32.03
Completeness [%]	100.0
Redundancy	13.1
CC(1/2)	1.000	0.746

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	295	PEG8000, MgAc