7XD8

Crystal Structure of Dengue Virus Serotype 2 (DENV2) Polymerase Elongation Complex (Native Form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-31
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9785
Spacegroup name	P 1 21 1
Unit cell lengths	141.728, 156.319, 174.424
Unit cell angles	90.00, 94.22, 90.00

Refinement procedure

Resolution	49.920 - 2.850
R-factor	0.2009
Rwork	0.199
R-free	0.24300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4k6m
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.950
High resolution limit [Å]	2.850	6.140	2.850
Rmerge	0.091	0.053	0.726
Rmeas	0.104	0.060	0.836
Rpim	0.049	0.028	0.401
Total number of observations	622366
Number of reflections	154806	14815	15980
<I/σ(I)>	7.4
Completeness [%]	87.9	82.8	90.9
Redundancy	4	4.2	3.9
CC(1/2)		0.996	0.742

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		289	Sodium formate, Glycerol