7XCN
Crystal structure of the MttB-MttC complex at 2.7 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE TPS 05A |
Synchrotron site | NSRRC |
Beamline | TPS 05A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-06-19 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.99984 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 120.434, 188.138, 123.952 |
Unit cell angles | 90.00, 119.25, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.700 |
Rwork | 0.192 |
R-free | 0.23820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ezx |
RMSD bond length | 0.007 |
RMSD bond angle | 1.253 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 20.000 | 20.000 | 2.750 |
High resolution limit [Å] | 2.700 | 7.220 | 2.700 |
Rmerge | 0.078 | 0.050 | 0.574 |
Rmeas | 0.092 | 0.060 | 0.682 |
Rpim | 0.049 | 0.033 | 0.364 |
Total number of observations | 458004 | ||
Number of reflections | 132103 | 6613 | 6619 |
<I/σ(I)> | 9.1 | ||
Completeness [%] | 99.8 | 98.3 | 100 |
Redundancy | 3.5 | 3.4 | 3.4 |
CC(1/2) | 0.982 | 0.730 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 283 | 0.5 ul MttB (3 mg/ml in 16mM potassium phosphate buffer pH7.4, 400mM NaCl, 160mM Imidazole, 20% glycerol, 1.6mM DTT) 0.5 ul MttC (10 mg/ml in 40 mM Tris buffer pH7, 200mM NaCl, 20% glycerol) 1 ul reservoir solution (18% PEG 8000, 0.1M citric acid pH 5, 1M LiC) |