7XCM
Crystal structure of sulfite MttB structure at 3.2 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2018-12-15 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54187 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 174.840, 174.840, 300.121 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 3.200 |
| Rwork | 0.167 |
| R-free | 0.24300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7xcl |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.277 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.270 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.196 | |
| Rpim | 0.254 | |
| Number of reflections | 74099 | 11486 |
| <I/σ(I)> | 2.6 | |
| Completeness [%] | 85.4 | |
| Redundancy | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 283 | 1 ul protein (8 mg/mL MttB in 20 mM potassium phosphate buffer pH 7.4, 500 mM NaCl, 240 mM imidazole, 20% glycerol), 1 ul reservoir (4% MPD, 0.1 M citric acid pH 4.5) solutions, reservoir solution containing saturated sodium dithionite |
