7XCB
Crystal structure of the mouse interleukin-9
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 11C |
Synchrotron site | PAL/PLS |
Beamline | 11C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-07-27 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 95.500, 95.500, 105.710 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 27.570 - 3.400 |
R-factor | 0.2436 |
Rwork | 0.241 |
R-free | 0.29030 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | AF-P15247-F1 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.490 |
High resolution limit [Å] | 3.400 | 3.400 |
Rmeas | 0.062 | 0.228 |
Number of reflections | 4255 | 297 |
<I/σ(I)> | 41.13 | |
Completeness [%] | 99.7 | 100 |
Redundancy | 19.82 | 18.55 |
CC(1/2) | 0.850 | 0.783 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 3.5 | 277.15 | 0.1M citric acid(pH 3.5), 2.0M Ammonium sulfate |