7XC8

Crystal structure of cotton alpha-like expansin GhEXLA1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	90
Detector technology	PIXEL
Collection date	2021-11-01
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9785
Spacegroup name	P 61 2 2
Unit cell lengths	151.273, 151.273, 61.026
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.880 - 2.550
R-factor	0.1961
Rwork	0.191
R-free	0.23930
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.994
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19rc5_4047)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.880	2.660
High resolution limit [Å]	2.550	2.550
Rmerge	0.124	0.867
Rmeas	0.127	0.888
Rpim	0.028	0.193
Number of reflections	13869	1663
<I/σ(I)>	33.3	6.3
Completeness [%]	99.8	100
Redundancy	38	39
CC(1/2)	1.000	0.963

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	291	PEG 3350, Tacsimate pH4.0