7XBT
Crystal structure of the adenylation domain of CmnG in complex with AMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE TPS 05A |
Synchrotron site | NSRRC |
Beamline | TPS 05A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2021-06-12 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 1 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 77.451, 77.451, 192.228 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.180 - 1.840 |
R-factor | 0.1985 |
Rwork | 0.197 |
R-free | 0.23280 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7xbs |
Data reduction software | HKL-2000 |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.910 |
High resolution limit [Å] | 1.840 | 1.840 |
Number of reflections | 51783 | 5095 |
<I/σ(I)> | 28.79 | 3.54 |
Completeness [%] | 99.5 | 100 |
Redundancy | 8.1 | 8.3 |
CC(1/2) | 0.977 | 0.902 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 30% w/v PEG-1000, and 100 mM HEPES, pH 7.5. |