7XBQ

Crystal structure of potato 14-3-3 protein St14f

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2017-10-13
Detector	RAYONIX MX225HE
Wavelength(s)	0.9000
Spacegroup name	C 1 2 1
Unit cell lengths	230.198, 105.281, 60.987
Unit cell angles	90.00, 104.84, 90.00

Refinement procedure

Resolution	47.580 - 2.450
R-factor	0.2123
Rwork	0.209
R-free	0.27680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3axy
RMSD bond length	0.012
RMSD bond angle	1.844
Data reduction software	HKL-2000
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.600	2.490
High resolution limit [Å]	2.450	2.450
Rmerge	0.063	0.442
Number of reflections	51160	2531
<I/σ(I)>	28.8
Completeness [%]	99.7
Redundancy	4.3
CC(1/2)	0.981

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG 8000, Ammonium tartrate, HEPES