7XBF
The complex structure of RshSTT182/200 RBD-insert2 bound to human ACE2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-10-25 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 0.979183 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.391, 123.891, 112.787 |
| Unit cell angles | 90.00, 93.26, 90.00 |
Refinement procedure
| Resolution | 40.630 - 3.510 |
| R-factor | 0.201 |
| Rwork | 0.199 |
| R-free | 0.23270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6lzg |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.498 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.650 |
| High resolution limit [Å] | 3.510 | 3.510 |
| Rmerge | 0.133 | 1.060 |
| Number of reflections | 28025 | 2812 |
| <I/σ(I)> | 9 | 1.1 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 4.8 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1M MES pH6.0, 0.2M magnesium chloride, 20% w/v PEG 6000 |
