7XAN
Structure of a triple-helix region of human collagen type III from Trautec
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-02-18 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.7749 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 15.783, 19.439, 83.164 |
Unit cell angles | 90.00, 93.77, 90.00 |
Refinement procedure
Resolution | 20.755 - 1.500 |
Rwork | 0.167 |
R-free | 0.19970 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2cuo |
RMSD bond length | 0.013 |
RMSD bond angle | 1.694 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.490 | 1.530 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.021 | 0.058 |
Number of reflections | 8076 | 360 |
<I/σ(I)> | 48.1 | |
Completeness [%] | 97.4 | |
Redundancy | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.04M K phosphate monobasic, 16% w/v PEG 8000, 20% v/v Glycerol |