7XAE
Crystal strucutre of PD-L1 and 3ONJA protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-01-18 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97852 |
Spacegroup name | I 41 |
Unit cell lengths | 103.079, 103.079, 173.710 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.560 - 3.440 |
R-factor | 0.2488 |
Rwork | 0.248 |
R-free | 0.26170 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3rrq |
RMSD bond length | 0.003 |
RMSD bond angle | 0.643 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.13-2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.550 |
High resolution limit [Å] | 3.430 | 3.430 |
Rmerge | 0.167 | 2.045 |
Number of reflections | 12131 | 12131 |
<I/σ(I)> | 18.797 | |
Completeness [%] | 82.5 | |
Redundancy | 13.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Sodium citrate tribasic dihydrate pH 5.5, 16% w/v Polyethylene glycol 8,000 |