7XA4

Thermotoga maritima ferritin variant-Tm-E (S111H) with Zn

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-03
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	1.276911
Spacegroup name	P 32 1 2
Unit cell lengths	55.840, 55.840, 129.392
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.190 - 1.960
R-factor	0.213
Rwork	0.210
R-free	0.23590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vlg
Data reduction software	XDS
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.190	2.030
High resolution limit [Å]	1.960	1.960
Number of reflections	33026	3210
<I/σ(I)>	10.06
Completeness [%]	98.2
Redundancy	2
CC(1/2)	0.978	0.874

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100mM Mes pH 6.0, 200mM Lithium sulfate, 2mM Zinc chloride, 35% MPD