7XA2

Thermotoga maritima ferritin variant-Tm-E(S111H)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-07
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	1.2769
Spacegroup name	P 32 1 2
Unit cell lengths	55.490, 55.490, 129.070
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.020 - 1.840
R-factor	0.2089
Rwork	0.207
R-free	0.22340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vlg
Data reduction software	xia2
Data scaling software	xia2
Phasing software	MOLREP
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.000	1.906
High resolution limit [Å]	1.840	1.840
Number of reflections	39447	3623
<I/σ(I)>	15.94
Completeness [%]	98.2
Redundancy	2
CC(1/2)	0.974	0.423

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100mM Mes pH 6.0, 200mM Lithium sulfate, 35% MPD