7X9X
Thermotoga Maritima ferritin variant-Tm-E(G40E) with Zn
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL02U1 |
Synchrotron site | SSRF |
Beamline | BL02U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-10-01 |
Detector | DECTRIS EIGER2 S 9M |
Wavelength(s) | 1.276911 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 55.500, 66.620, 92.340 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.560 - 2.300 |
R-factor | 0.1957 |
Rwork | 0.190 |
R-free | 0.25110 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1vlg |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.560 | 2.382 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 31502 | 3042 |
<I/σ(I)> | 5.9 | |
Completeness [%] | 99.7 | |
Redundancy | 1.9 | |
CC(1/2) | 0.997 | 0.699 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 25mM HEPES pH 8.0, 35% MPD, 2mM Zinc chloride |