7X9R

Crystal structure of the antirepressor GmaR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2018-04-11
Detector	ADSC QUANTUM 270
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	74.736, 85.779, 104.310
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.590 - 2.250
R-factor	0.2255
Rwork	0.224
R-free	0.26070
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	0.614
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AutoSol
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.290
High resolution limit [Å]	2.250	2.250
Number of reflections	32149	1542
<I/σ(I)>	19.7	1.3
Completeness [%]	99.3	97.5
Redundancy	3.7	3.4
CC(1/2)	0.991	0.495

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	sodium acetate, sodium cacodylate