7X9N
Crystal structure of MutT-8-oxo-dGTP complex with three Mg2+ ions: Reaction using Mg2+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-1A |
Synchrotron site | Photon Factory |
Beamline | BL-1A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-02-14 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 38.348, 55.931, 59.079 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.630 - 1.700 |
R-factor | 0.1623 |
Rwork | 0.160 |
R-free | 0.20370 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 3a6t |
RMSD bond length | 0.006 |
RMSD bond angle | 1.341 |
Data reduction software | HKL-2000 |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.630 | 1.730 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.301 | |
Number of reflections | 14231 | 610 |
<I/σ(I)> | 17.1 | |
Completeness [%] | 98.2 | |
Redundancy | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 288 | potassium sodium tartrate, sodium citrate, ammonium sulfate |