7X9K
Crystal structure of MutT-8-oxo-dGTP complex: Reaction for 2 hr using 10 mM Mn2+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-10-01 |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 38.150, 55.984, 59.135 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.530 - 1.810 |
R-factor | 0.1573 |
Rwork | 0.156 |
R-free | 0.18190 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 3a6t |
RMSD bond length | 0.006 |
RMSD bond angle | 1.385 |
Data reduction software | HKL-2000 |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.530 | 1.870 |
High resolution limit [Å] | 1.810 | 1.810 |
Rmerge | 0.065 | 0.321 |
Number of reflections | 11882 | 1043 |
<I/σ(I)> | 22.9 | |
Completeness [%] | 98.5 | |
Redundancy | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 288 | potassium sodium tartrate, sodium citrate, ammonium sulfate |