7X9K

Crystal structure of MutT-8-oxo-dGTP complex: Reaction for 2 hr using 10 mM Mn2+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Collection date	2005-10-01
Detector	RIGAKU JUPITER 210
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	38.150, 55.984, 59.135
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.530 - 1.810
R-factor	0.1573
Rwork	0.156
R-free	0.18190
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	3a6t
RMSD bond length	0.006
RMSD bond angle	1.385
Data reduction software	HKL-2000
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.530	1.870
High resolution limit [Å]	1.810	1.810
Rmerge	0.065	0.321
Number of reflections	11882	1043
<I/σ(I)>	22.9
Completeness [%]	98.5
Redundancy	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		288	potassium sodium tartrate, sodium citrate, ammonium sulfate