7X9J

Crystal structure of MutT-8-oxo-dGTP complex: Reaction for 24 hr using 5 mM Mn2+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Collection date	2005-03-28
Detector	RIGAKU JUPITER 210
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	38.130, 55.980, 59.198
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.170 - 1.980
R-factor	0.1549
Rwork	0.152
R-free	0.21240
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	3a6t
RMSD bond length	0.006
RMSD bond angle	1.149
Data reduction software	HKL-2000
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.170	2.050
High resolution limit [Å]	1.980	1.980
Rmerge	0.052	0.133
Number of reflections	9248	809
<I/σ(I)>	52.7
Completeness [%]	99.3
Redundancy	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		288	potassium sodium tartrate, sodium citrate, ammonium sulfate