7X9H
Crystal structure of MutT-8-oxo-dGTP complex: Reaction for 9 hr using 5 mM Mn2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-03-22 |
| Detector | Bruker DIP-6040 |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.095, 55.923, 59.169 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.030 - 1.690 |
| R-factor | 0.1622 |
| Rwork | 0.160 |
| R-free | 0.20460 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3a6t |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.357 |
| Data reduction software | HKL-2000 |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.030 | 1.760 |
| High resolution limit [Å] | 1.690 | 1.690 |
| Rmerge | 0.095 | 0.259 |
| Number of reflections | 14294 | 1196 |
| <I/σ(I)> | 25.1 | |
| Completeness [%] | 97.5 | |
| Redundancy | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 288 | potassium sodium tartrate, sodium citrate, ammonium sulfate |
