7X99
Anabolic ornithine carbamoyltransferases (OTCs) from Psychrobacter sp. PAMC 21119
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2021-07-13 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9795 |
Spacegroup name | P 63 |
Unit cell lengths | 129.610, 129.610, 75.200 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.760 - 2.200 |
Rwork | 0.183 |
R-free | 0.23520 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ort |
RMSD bond length | 0.009 |
RMSD bond angle | 1.596 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.760 | 2.270 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 1.326 | |
Number of reflections | 36629 | 3171 |
<I/σ(I)> | 25.9 | |
Completeness [%] | 100.0 | |
Redundancy | 20.9 | |
CC(1/2) | 1.000 | 1.000 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1M Sodium Acetate: HCl, pH 4.6, 1M Ammonium Citrate Dibasic |