7X8Q

Frizzled-10 CRD in complex with F10_A9 Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	P 41 21 2
Unit cell lengths	156.452, 156.452, 94.294
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.880 - 2.650
R-factor	0.2071
Rwork	0.205
R-free	0.24730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5cm4
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.880	2.700
High resolution limit [Å]	2.650	2.650
Rmerge	0.105	0.878
Rmeas	0.108	0.905
Rpim	0.026	0.217
Number of reflections	34538	1698
<I/σ(I)>	29.2	3.75
Completeness [%]	100.0	100
Redundancy	16.6	16.9
CC(1/2)	0.997	0.899

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	295	0.1 M HEPES, pH 7.0, 30% Jeffamine ED-2001, pH 7.0