7X85
Crystal structure of chicken CENP-C Cupin domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-01-20 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 0.99999 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 112.587, 112.587, 212.110 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.558 - 2.639 |
Rwork | 0.185 |
R-free | 0.25170 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2vpv |
RMSD bond length | 0.006 |
RMSD bond angle | 1.236 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | BUCCANEER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.750 | 48.750 | 2.770 |
High resolution limit [Å] | 2.639 | 8.750 | 2.640 |
Rmerge | 0.112 | 0.025 | 2.821 |
Rmeas | 0.118 | 0.027 | 2.963 |
Rpim | 0.036 | 0.009 | 0.902 |
Number of reflections | 24156 | 790 | 3093 |
<I/σ(I)> | 17.8 | ||
Completeness [%] | 99.8 | ||
Redundancy | 19.3 | 14.9 | 19.9 |
CC(1/2) | 0.999 | 0.999 | 0.735 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.2 M lithium citrate, 0.1 M MES-NaOH pH 6.5 and 20% PEG4000 |