7X7W
The X-ray Crystallographic Structure of D-Psicose 3-epimerase from Clostridia bacterium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-12-09 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97915 |
Spacegroup name | I 1 2 1 |
Unit cell lengths | 60.018, 70.657, 153.784 |
Unit cell angles | 90.00, 99.32, 90.00 |
Refinement procedure
Resolution | 37.939 - 2.097 |
R-factor | 0.2029 |
Rwork | 0.200 |
R-free | 0.25420 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3vnk |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX (1.14_3260) |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.950 | 2.160 |
High resolution limit [Å] | 2.090 | 2.100 |
Rmerge | 0.032 | 0.124 |
Number of reflections | 37135 | 2994 |
<I/σ(I)> | 30.4 | |
Completeness [%] | 99.4 | |
Redundancy | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M Succine acid pH 7.0, 0.1 M HEPES pH7.0, 1% w/v PEG MME 2000 |