7X7I

Ancestral L-Lys oxidase (AncLLysO-2) ligand free form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-01
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	79.246, 76.533, 92.708
Unit cell angles	90.00, 104.39, 90.00

Refinement procedure

Resolution	44.900 - 1.550
Rwork	0.163
R-free	0.19100
Structure solution method	SAD
RMSD bond length	0.013
RMSD bond angle	1.775
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	AutoSol
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.900	1.630
High resolution limit [Å]	1.550	1.550
Number of reflections	155511	22407
<I/σ(I)>	12.6
Completeness [%]	99.8
Redundancy	6.6
CC(1/2)	0.998	0.952

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.2 M Magnesium chloride hexahydrate, 0.1 M HEPES pH 7.5, 25% w/v Polyethylene glycol 3,350, 0.1 M NDSB-256