7X7E

SARS-CoV-2 RBD and Nb22

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-09
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97915
Spacegroup name	P 21 21 21
Unit cell lengths	73.790, 88.684, 172.475
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.230 - 2.670
R-factor	0.2069
Rwork	0.204
R-free	0.24950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7cjf
RMSD bond length	0.003
RMSD bond angle	0.544
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.750
High resolution limit [Å]	2.670	2.670
Number of reflections	32672	1579
<I/σ(I)>	12.3
Completeness [%]	99.8
Redundancy	7.2
CC(1/2)	0.999	0.763

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	290	0.1 M HEPES (pH 480 7.0), 5% MPD, 10% PEG 10000