7X77

Ectodomain structure of per os infectivity factor 5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-20
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9789
Spacegroup name	P 21 21 2
Unit cell lengths	68.173, 92.360, 52.646
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.180 - 2.200
R-factor	0.2011
Rwork	0.199
R-free	0.24400
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.771
Data reduction software	XDS
Phasing software	autoSHARP
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.180	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.139	0.455
Number of reflections	17363	1673
<I/σ(I)>	12.94
Completeness [%]	99.8
Redundancy	12.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	289	0.1 M BICINE, 25% PEG3350