7X5G
Nrf2 (A510Y)-MafG heterodimer bound with CsMBE2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-06-03 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 246.022, 55.018, 86.152 |
Unit cell angles | 90.00, 97.30, 90.00 |
Refinement procedure
Resolution | 33.890 - 2.300 |
R-factor | 0.222 |
Rwork | 0.219 |
R-free | 0.24980 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3a5t |
RMSD bond length | 0.003 |
RMSD bond angle | 0.454 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.340 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.037 | 0.520 |
Number of reflections | 50434 | 2456 |
<I/σ(I)> | 34.2 | 2.7 |
Completeness [%] | 98.3 | 96.5 |
Redundancy | 4.6 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 298 | 50 mM Tris-HCl (pH 7.5), 100 mM KCl, 10% polyethylene glycol monomethyl ether 550, 2% (v/v) glycerol |