7X5D
Crystal Structure of the K316C mutant of Human Lamin A/C Coil 2 (residues 244-340)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-07-14 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 29.788, 40.966, 140.714 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.100 - 1.820 |
R-factor | 0.2409 |
Rwork | 0.239 |
R-free | 0.27920 |
Structure solution method | AB INITIO PHASING |
Data scaling software | HKL-2000 |
Phasing software | Arcimboldo |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 20.100 | 20.000 | 1.830 |
High resolution limit [Å] | 1.800 | 4.860 | 1.800 |
Rmerge | 0.062 | 0.050 | 0.334 |
Rmeas | 0.066 | 0.053 | 0.355 |
Rpim | 0.020 | 0.017 | 0.117 |
Number of reflections | 15449 | 913 | 541 |
<I/σ(I)> | 12 | ||
Completeness [%] | 95.1 | 99.1 | 68.4 |
Redundancy | 9.5 | 12 | 6.8 |
CC(1/2) | 0.998 | 0.939 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 287 | Tacsimate (pH 5.5), PEG 3350, methyl mercury acetate |